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Assay Detail

CHEMBL4418880

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal MBP tagged human neuraminidase-2 expressed in Escherichia coli pre-incubated for 15 mins before 4MU-NANA substrate addition and measured every 30 secs for 30 mins by fluorescence based assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sialidase-2 (CHEMBL3200)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Methods of preventing or treating atherosclerosis with inhibitors of specific isoenzymes of human neuraminidase
(2018)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 4.73 5.24

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL222813 ZANAMIVIR 4.0 1 5.24
CHEMBL4099818 1 4.77
CHEMBL96712 NEU5AC2EN 1 4.60
CHEMBL4076203 1 4.32
CHEMBL1289650 1 -
CHEMBL4440333 1 -
CHEMBL4291908 1 -
CHEMBL4076483 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL222813 ZANAMIVIR Ki = 5700.0 nM 5.24
CHEMBL4099818 Ki = 17000.0 nM 4.77
CHEMBL96712 NEU5AC2EN Ki = 25000.0 nM 4.60
CHEMBL4076203 Ki = 48000.0 nM 4.32
CHEMBL1289650 Ki - -
CHEMBL4440333 Ki - -
CHEMBL4291908 Ki - -
CHEMBL4076483 Ki - -