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Assay Detail

CHEMBL4423277

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ABCB1 in human A2780/ADR cells preincubated for 30 mins followed by calcein AM addition and measured every 60 secs for 1 hr by fluorescence assay
16
Total Activities
15
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

ATP-dependent translocase ABCB1 (CHEMBL4302)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel chalcone and flavone derivatives as selective and dual inhibitors of the transport proteins ABCB1 and ABCG2.
Silbermann K, Shah CP, Sahu NU, Juvale K, Stefan SM, Kharkar PS, Wiese M.
Eur J Med Chem (2019) 164 : 193 -213
PubMed 30594677 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 5.41 5.85
Imax 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4474709 2 5.85
CHEMBL4462468 1 5.72
CHEMBL4470070 1 5.30
CHEMBL4540332 1 5.26
CHEMBL4210110 1 4.93
CHEMBL4524909 1 -
CHEMBL4528836 1 -
CHEMBL4567676 1 -
CHEMBL4451229 1 -
CHEMBL4583169 1 -
CHEMBL4205213 1 -
CHEMBL4555106 1 -
CHEMBL4463976 1 -
CHEMBL4583488 1 -
CHEMBL4440031 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4474709 IC50 = 1410.0 nM 5.85
CHEMBL4462468 IC50 = 1890.0 nM 5.72
CHEMBL4470070 IC50 = 5010.0 nM 5.30
CHEMBL4540332 IC50 = 5430.0 nM 5.26
CHEMBL4210110 IC50 = 11700.0 nM 4.93
CHEMBL4524909 IC50 - -
CHEMBL4474709 Imax = 51.0 % -
CHEMBL4528836 IC50 - -
CHEMBL4567676 IC50 - -
CHEMBL4451229 IC50 - -
CHEMBL4583169 IC50 - -
CHEMBL4205213 IC50 - -
CHEMBL4555106 IC50 - -
CHEMBL4463976 IC50 - -
CHEMBL4583488 IC50 - -
CHEMBL4440031 IC50 - -