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Assay Detail

CHEMBL4424491

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NS5A in HCV genotype 3a assessed as decrease in viral replication
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Hepatitis C virus subtype 3a
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Nonstructural protein 5A (CHEMBL3307224)
Type SINGLE PROTEIN
Organism Hepatitis C virus

Publication

Discovery of novel pan-genotypic HCV NS5A inhibitors containing a novel tetracyclic core.
Yu W, Hu B, Zhong B, Hao J, Lei Z, Agrawal S, Rokosz L, Liu R, Chen S, Asante-Appiah E, Kozlowski JA.
Bioorg Med Chem Lett (2019) 29 : 700 -706
PubMed 30711390 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 26 9.70 10.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3039514 ELBASVIR 4.0 1 10.70
CHEMBL4552270 1 10.70
CHEMBL4452034 1 10.59
CHEMBL4524514 1 10.52
CHEMBL4575633 1 10.40
CHEMBL4580295 1 10.22
CHEMBL4560981 1 10.22
CHEMBL3808674 1 10.15
CHEMBL4566096 1 10.10
CHEMBL4543823 1 9.70
CHEMBL4582225 1 9.24
CHEMBL4461377 1 9.14
CHEMBL4542548 1 9.14
CHEMBL4578481 1 8.96
CHEMBL4577346 1 8.96
CHEMBL4440064 1 8.85
CHEMBL4450175 1 8.77
CHEMBL4461150 1 8.24
CHEMBL4533911 1 -
CHEMBL4454064 1 -
CHEMBL4461797 1 -
CHEMBL4569232 1 -
CHEMBL4577499 1 -
CHEMBL4527728 1 -
CHEMBL4438612 1 -
CHEMBL3360472 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3039514 ELBASVIR EC50 = 0.02 nM 10.70
CHEMBL4552270 EC50 = 0.02 nM 10.70
CHEMBL4452034 EC50 = 0.026 nM 10.59
CHEMBL4524514 EC50 = 0.03 nM 10.52
CHEMBL4575633 EC50 = 0.04 nM 10.40
CHEMBL4580295 EC50 = 0.06 nM 10.22
CHEMBL4560981 EC50 = 0.06 nM 10.22
CHEMBL3808674 EC50 = 0.07 nM 10.15
CHEMBL4566096 EC50 = 0.08 nM 10.10
CHEMBL4543823 EC50 = 0.2 nM 9.70
CHEMBL4582225 EC50 = 0.58 nM 9.24
CHEMBL4461377 EC50 = 0.72 nM 9.14
CHEMBL4542548 EC50 = 0.72 nM 9.14
CHEMBL4578481 EC50 = 1.1 nM 8.96
CHEMBL4577346 EC50 = 1.1 nM 8.96
CHEMBL4440064 EC50 = 1.4 nM 8.85
CHEMBL4450175 EC50 = 1.7 nM 8.77
CHEMBL4461150 EC50 = 5.8 nM 8.24
CHEMBL4533911 EC50 - -
CHEMBL4454064 EC50 - -
CHEMBL4461797 EC50 > 1.0 nM -
CHEMBL4569232 EC50 - -
CHEMBL4577499 EC50 > 1.0 nM -
CHEMBL4527728 EC50 > 1.0 nM -
CHEMBL4438612 EC50 > 1.0 nM -
CHEMBL3360472 EC50 - -