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Assay Detail

CHEMBL4425914

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ERK in PMA-stimulated human HepG2 cells assessed as inhibition of p90RSK serine-380 phosphorylation
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HepG2
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery of (S)-1-(1-(4-Chloro-3-fluorophenyl)-2-hydroxyethyl)-4-(2-((1-methyl-1H-pyrazol-5-yl)amino)pyrimidin-4-yl)pyridin-2(1H)-one (GDC-0994), an Extracellular Signal-Regulated Kinase 1/2 (ERK1/2) Inhibitor in Early Clinical Development.
Blake JF, Burkard M, Chan J, Chen H, Chou KJ, Diaz D, Dudley DA, Gaudino JJ, Gould SE, Grina J, Hunsaker T, Liu L, Martinson M, Moreno D, Mueller L, Orr C, Pacheco P, Qin A, Rasor K, Ren L, Robarge K, Shahidi-Latham S, Stults J, Sullivan F, Wang W, Yin J, Zhou A, Belvin M, Merchant M, Moffat J, Schwarz JB.
J Med Chem (2016) 59 : 5650 -5660
PubMed 27227380 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 6.65 8.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4571067 1 8.10
CHEMBL3544964 RAVOXERTINIB 2.0 1 7.92
CHEMBL4436718 1 7.47
CHEMBL4565958 1 7.42
CHEMBL4435650 1 7.36
CHEMBL3409588 1 7.31
CHEMBL4114404 1 7.19
CHEMBL3675721 1 7.00
CHEMBL4474917 1 6.90
CHEMBL3675719 1 6.89
CHEMBL4459138 1 6.88
CHEMBL4460988 1 6.64
CHEMBL4452981 1 6.61
CHEMBL4450215 1 6.53
CHEMBL4592894 1 6.28
CHEMBL4434981 1 6.20
CHEMBL4461076 1 5.60
CHEMBL4446409 1 5.46
CHEMBL4458128 1 5.30
CHEMBL4463669 1 5.30
CHEMBL4527526 1 5.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4571067 IC50 = 8.0 nM 8.10
CHEMBL3544964 RAVOXERTINIB IC50 = 12.0 nM 7.92
CHEMBL4436718 IC50 = 34.0 nM 7.47
CHEMBL4565958 IC50 = 38.0 nM 7.42
CHEMBL4435650 IC50 = 44.0 nM 7.36
CHEMBL3409588 IC50 = 49.0 nM 7.31
CHEMBL4114404 IC50 = 64.0 nM 7.19
CHEMBL3675721 IC50 = 100.0 nM 7.00
CHEMBL4474917 IC50 = 127.0 nM 6.90
CHEMBL3675719 IC50 = 129.0 nM 6.89
CHEMBL4459138 IC50 = 132.0 nM 6.88
CHEMBL4460988 IC50 = 230.0 nM 6.64
CHEMBL4452981 IC50 = 247.0 nM 6.61
CHEMBL4450215 IC50 = 298.0 nM 6.53
CHEMBL4592894 IC50 = 530.0 nM 6.28
CHEMBL4434981 IC50 = 635.0 nM 6.20
CHEMBL4461076 IC50 = 2500.0 nM 5.60
CHEMBL4446409 IC50 = 3500.0 nM 5.46
CHEMBL4458128 IC50 = 5000.0 nM 5.30
CHEMBL4463669 IC50 = 5000.0 nM 5.30
CHEMBL4527526 IC50 = 5000.0 nM 5.30