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Assay Detail

CHEMBL4426118

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of carbonic anhydrase 9 in human HT-29 cells assessed as reduction in cell viability in presence of CoCl2-induced hypoxic conditions after 72 hrs by cell counter method
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HT-29
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Carbonic anhydrase 9 (CHEMBL3594)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

1,2-Benzisothiazole Derivatives Bearing 4-, 5-, or 6-Alkyl/arylcarboxamide Moieties Inhibit Carbonic Anhydrase Isoform IX (CAIX) and Cell Proliferation under Hypoxic Conditions.
Coviello V, Marchi B, Sartini S, Quattrini L, Marini AM, Simorini F, Taliani S, Salerno S, Orlandi P, Fioravanti A, Desidero TD, Vullo D, Da Settimo F, Supuran CT, Bocci G, La Motta C.
J Med Chem (2016) 59 : 6547 -6552
PubMed 27305384 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 4.33 4.36

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4532693 1 4.36
CHEMBL4584482 1 4.29
CHEMBL4545838 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4532693 IC50 = 44100.0 nM 4.36
CHEMBL4584482 IC50 = 50970.0 nM 4.29
CHEMBL4545838 IC50 > 100000.0 nM -