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Assay Detail

CHEMBL4426453

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ERK in human A375SM cells harboring BRAF V600E mutant assessed as inhibition of RSK phosphorylation measured after 4 hrs by mesoscale electrochemiluminescent sandwich assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery of 1-(1H-Pyrazolo[4,3-c]pyridin-6-yl)urea Inhibitors of Extracellular Signal-Regulated Kinase (ERK) for the Treatment of Cancers.
Lim J, Kelley EH, Methot JL, Zhou H, Petrocchi A, Chen H, Hill SE, Hinton MC, Hruza A, Jung JO, Maclean JK, Mansueto M, Naumov GN, Philippar U, Raut S, Spacciapoli P, Sun D, Siliphaivanh P.
J Med Chem (2016) 59 : 6501 -6511
PubMed 27329786 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 6.56 7.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2408789 1 7.16
CHEMBL3663084 1 7.01
CHEMBL3654680 1 6.96
CHEMBL3654682 1 6.89
CHEMBL2408788 1 6.66
CHEMBL3663099 1 6.52
CHEMBL3654673 1 6.46
CHEMBL3654681 1 6.44
CHEMBL3654676 1 6.26
CHEMBL3654729 1 6.12
CHEMBL3663095 1 6.09
CHEMBL3654742 1 6.09
CHEMBL3654712 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2408789 IC50 = 70.0 nM 7.16
CHEMBL3663084 IC50 = 97.0 nM 7.01
CHEMBL3654680 IC50 = 110.0 nM 6.96
CHEMBL3654682 IC50 = 130.0 nM 6.89
CHEMBL2408788 IC50 = 220.0 nM 6.66
CHEMBL3663099 IC50 = 300.0 nM 6.52
CHEMBL3654673 IC50 = 350.0 nM 6.46
CHEMBL3654681 IC50 = 360.0 nM 6.44
CHEMBL3654676 IC50 = 550.0 nM 6.26
CHEMBL3654729 IC50 = 750.0 nM 6.12
CHEMBL3663095 IC50 = 810.0 nM 6.09
CHEMBL3654742 IC50 = 810.0 nM 6.09
CHEMBL3654712 IC50 > 10000.0 nM -