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Assay Detail

CHEMBL4427015

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of V-tagged mouse DAGLbeta expressed in HEK2934T cell membrane fractions using SAG as substrate assessed as accumulation of 2-AG preincubated for 30 mins followed by probe addition measured after 30 mins by LC-MS natural substrate assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Diacylglycerol lipase-beta (CHEMBL5656)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Inhibitors of diacylglycerol lipases in neurodegenerative and metabolic disorders.
Janssen FJ, van der Stelt M.
Bioorg Med Chem Lett (2016) 26 : 3831 -3837
PubMed 27394666 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 7.10 7.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2144065 1 7.10
CHEMBL2136920 1 7.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2144065 IC50 = 79.43 nM 7.10
CHEMBL2136920 IC50 = 79.43 nM 7.10