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Assay Detail

CHEMBL4427221

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at human recombinant P2X7 receptor expressed in 1321N1 cells assessed as inhibition of BzATP-induced calcium mobilization incubated for 30 mins measured after 180 seconds by calcium-4 dye based FLIPR assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

P2X purinoceptor 7 (CHEMBL4805)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The evolution of P2X7 antagonists with a focus on CNS indications.
Rech JC, Bhattacharya A, Letavic MA, Savall BM.
Bioorg Med Chem Lett (2016) 26 : 3838 -3845
PubMed 27426304 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 8.01 8.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4471289 1 8.62
CHEMBL4442874 1 8.57
CHEMBL4465051 1 8.35
CHEMBL4546974 1 8.16
CHEMBL4450832 1 8.07
CHEMBL3674061 1 8.07
CHEMBL4077415 1 8.06
CHEMBL4567755 1 7.96
CHEMBL3674077 1 7.96
CHEMBL3674106 1 7.72
CHEMBL3674060 1 6.62

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4471289 IC50 = 2.4 nM 8.62
CHEMBL4442874 IC50 = 2.7 nM 8.57
CHEMBL4465051 IC50 = 4.5 nM 8.35
CHEMBL4546974 IC50 = 6.9 nM 8.16
CHEMBL4450832 IC50 = 8.5 nM 8.07
CHEMBL3674061 IC50 = 8.5 nM 8.07
CHEMBL4077415 IC50 = 8.7 nM 8.06
CHEMBL4567755 IC50 = 11.0 nM 7.96
CHEMBL3674077 IC50 = 11.0 nM 7.96
CHEMBL3674106 IC50 = 19.0 nM 7.72
CHEMBL3674060 IC50 = 240.0 nM 6.62