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Assay Detail

CHEMBL4427760

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human IDO1 expressed in Escherichia coli BL21 Star assessed as kynurenine formation using L-Trp as substrate preincubated for 90 mins measured after 30 mins by bridge-IT fluorescence assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Indoleamine 2,3-dioxygenase 1 (CHEMBL4685)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

1,2,3-Triazoles as inhibitors of indoleamine 2,3-dioxygenase 2 (IDO2).
Röhrig UF, Majjigapu SR, Caldelari D, Dilek N, Reichenbach P, Ascencao K, Irving M, Coukos G, Vogel P, Zoete V, Michielin O.
Bioorg Med Chem Lett (2016) 26 : 4330 -4333
PubMed 27469130 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 4.46 4.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL504816 (L)-1-METHYLTRYPTOPHAN 1 4.46

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL504816 (L)-1-METHYLTRYPTOPHAN IC50 = 35000.0 nM 4.46