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Assay Detail

CHEMBL4427798

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Binding
Inhibition of human CENP-E motor domain assessed as ADP levels preincubated with protein motor domain/microtubule for 60 mins followed by ATP addition measured after 20 mins by ADPglo assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Centromere-associated protein E (CHEMBL5870)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and synthesis of fused bicyclic inhibitors targeting the L5 loop site of centromere-associated protein E.
Hirayama T, Okaniwa M, Banno H, Kakei H, Ohashi A, Ohori M, Nambu T, Iwai K, Kawamoto T, Yokota A, Miyamoto M, Ishikawa T.
Bioorg Med Chem Lett (2016) 26 : 4296 -4300
PubMed 27476141 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 8.35 8.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4470356 1 8.82
CHEMBL4579540 1 8.80
CHEMBL4549646 1 8.77
CHEMBL4468555 1 8.66
CHEMBL4515745 1 8.57
CHEMBL4579162 1 7.85
CHEMBL4466617 1 7.72
CHEMBL4483703 1 7.62

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4470356 IC50 = 1.5 nM 8.82
CHEMBL4579540 IC50 = 1.6 nM 8.80
CHEMBL4549646 IC50 = 1.7 nM 8.77
CHEMBL4468555 IC50 = 2.2 nM 8.66
CHEMBL4515745 IC50 = 2.7 nM 8.57
CHEMBL4579162 IC50 = 14.0 nM 7.85
CHEMBL4466617 IC50 = 19.0 nM 7.72
CHEMBL4483703 IC50 = 24.0 nM 7.62