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Assay Detail

CHEMBL4428660

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of TAFMT from human pancreatic N-terminal His6-tagged glucokinase isoform 1 expressed in Escherichia coli BL21(DE3) by stopped-flow fluorometric method
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Hexokinase-4 (CHEMBL3820)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Molecular Dynamics Simulations and Kinetic Measurements to Estimate and Predict Protein-Ligand Residence Times.
Mollica L, Theret I, Antoine M, Perron-Sierra F, Charton Y, Fourquez JM, Wierzbicki M, Boutin JA, Ferry G, Decherchi S, Bottegoni G, Ducrot P, Cavalli A.
J Med Chem (2016) 59 : 7167 -7176
PubMed 27391254 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 6 5.86 6.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4483049 1 6.40
CHEMBL4534484 1 6.14
CHEMBL1783734 PIRAGLIATIN 2.0 1 6.08
CHEMBL4522750 1 5.70
CHEMBL205627 1 5.48
CHEMBL4457828 1 5.36

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4483049 Kd = 400.0 nM 6.40
CHEMBL4534484 Kd = 720.0 nM 6.14
CHEMBL1783734 PIRAGLIATIN Kd = 830.0 nM 6.08
CHEMBL4522750 Kd = 2000.0 nM 5.70
CHEMBL205627 Kd = 3300.0 nM 5.48
CHEMBL4457828 Kd = 4400.0 nM 5.36