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Assay Detail

CHEMBL4428712

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Functional
Inhibition of cell proliferation of human B cells pre-incubated for 30 mins before anti-IgM/CD40L stimulation and measured after 72 hrs by [3H]thymidine incorporation assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type B cell
Confidence 1 — Organism
Curated By Autocuration

Target

B cell (CHEMBL5314312)
Type CELL-LINE
Organism Homo sapiens

Publication

Discovery and in Vivo Evaluation of the Potent and Selective PI3Kδ Inhibitors 2-((1S)-1-((6-Amino-5-cyano-4-pyrimidinyl)amino)ethyl)-6-fluoro-N-methyl-3-(2-pyridinyl)-4-quinolinecarboxamide (AM-0687) and 2-((1S)-1-((6-Amino-5-cyano-4-pyrimidinyl)amino)ethyl)-5-fluoro-N-methyl-3-(2-pyridinyl)-4-quinolinecarboxamide (AM-1430).
Gonzalez-Lopez de Turiso F, Hao X, Shin Y, Bui M, Campuzano ID, Cardozo M, Dunn MC, Duquette J, Fisher B, Foti RS, Henne K, He X, Hu YL, Kelly RC, Johnson MG, Lucas BS, McCarter J, McGee LR, Medina JC, Metz D, San Miguel T, Mohn D, Tran T, Vissinga C, Wannberg S, Whittington DA, Whoriskey J, Yu G, Zalameda L, Zhang X, Cushing TD.
J Med Chem (2016) 59 : 7252 -7267
PubMed 27411843 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 8.75 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4443843 1 9.70
CHEMBL4470291 1 9.10
CHEMBL4449576 1 8.92
CHEMBL4525494 1 8.92
CHEMBL4464055 1 8.92
CHEMBL4461539 1 8.89
CHEMBL4572863 1 8.80
CHEMBL4442808 1 8.80
CHEMBL4555334 1 8.74
CHEMBL4516601 1 8.72
CHEMBL4570159 1 8.66
CHEMBL4438514 1 8.54
CHEMBL3403678 1 8.34
CHEMBL4594095 1 8.13
CHEMBL4303596 AMG-319 2.0 1 8.07
CHEMBL4449366 1 -
CHEMBL4542785 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4443843 IC50 = 0.2 nM 9.70
CHEMBL4470291 IC50 = 0.8 nM 9.10
CHEMBL4449576 IC50 = 1.2 nM 8.92
CHEMBL4525494 IC50 = 1.2 nM 8.92
CHEMBL4464055 IC50 = 1.2 nM 8.92
CHEMBL4461539 IC50 = 1.3 nM 8.89
CHEMBL4572863 IC50 = 1.6 nM 8.80
CHEMBL4442808 IC50 = 1.6 nM 8.80
CHEMBL4555334 IC50 = 1.8 nM 8.74
CHEMBL4516601 IC50 = 1.9 nM 8.72
CHEMBL4570159 IC50 = 2.2 nM 8.66
CHEMBL4438514 IC50 = 2.9 nM 8.54
CHEMBL3403678 IC50 = 4.6 nM 8.34
CHEMBL4594095 IC50 = 7.4 nM 8.13
CHEMBL4303596 AMG-319 IC50 = 8.6 nM 8.07
CHEMBL4449366 IC50 - -
CHEMBL4542785 IC50 - -