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Assay Detail

CHEMBL4429476

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal GST-tagged FLT3 (564 to end residues) expressed by baculovirus in Sf21 insect cells measured after 10 mins in presence of [gamma-33P]ATP by scintillation counter method
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf21
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Receptor-type tyrosine-protein kinase FLT3 (CHEMBL1974)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Drug Discovery against Psoriasis: Identification of a New Potent FMS-like Tyrosine Kinase 3 (FLT3) Inhibitor, 1-(4-((1H-Pyrazolo[3,4-d]pyrimidin-4-yl)oxy)-3-fluorophenyl)-3-(5-(tert-butyl)isoxazol-3-yl)urea, That Showed Potent Activity in a Psoriatic Animal Model.
Li GB, Ma S, Yang LL, Ji S, Fang Z, Zhang G, Wang LJ, Zhong JM, Xiong Y, Wang JH, Huang SZ, Li LL, Xiang R, Niu D, Chen YC, Yang SY.
J Med Chem (2016) 59 : 8293 -8305
PubMed 27535613 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 7.44 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4444648 1 8.70
CHEMBL4519741 1 8.30
CHEMBL4448466 1 8.22
CHEMBL4514229 1 8.22
CHEMBL4435299 1 8.10
CHEMBL4435097 1 8.10
CHEMBL4469122 1 8.00
CHEMBL4519896 1 7.96
CHEMBL4528431 1 7.57
CHEMBL4460440 1 7.55
CHEMBL4475895 1 7.44
CHEMBL4440184 1 7.42
CHEMBL2332840 1 7.41
CHEMBL4444689 1 7.38
CHEMBL4523041 1 7.30
CHEMBL4591330 1 7.28
CHEMBL4438387 1 7.28
CHEMBL4451381 1 7.25
CHEMBL4470513 1 7.21
CHEMBL4474529 1 7.18
CHEMBL4443351 1 7.14
CHEMBL4471087 1 7.10
CHEMBL4550097 1 7.09
CHEMBL4544397 1 6.66
CHEMBL4435018 1 6.53
CHEMBL4530061 1 6.35
CHEMBL4475159 1 6.18

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4444648 IC50 = 2.0 nM 8.70
CHEMBL4519741 IC50 = 5.0 nM 8.30
CHEMBL4448466 IC50 = 6.0 nM 8.22
CHEMBL4514229 IC50 = 6.0 nM 8.22
CHEMBL4435299 IC50 = 8.0 nM 8.10
CHEMBL4435097 IC50 = 8.0 nM 8.10
CHEMBL4469122 IC50 = 10.0 nM 8.00
CHEMBL4519896 IC50 = 11.0 nM 7.96
CHEMBL4528431 IC50 = 27.0 nM 7.57
CHEMBL4460440 IC50 = 28.0 nM 7.55
CHEMBL4475895 IC50 = 36.0 nM 7.44
CHEMBL4440184 IC50 = 38.0 nM 7.42
CHEMBL2332840 IC50 = 39.0 nM 7.41
CHEMBL4444689 IC50 = 42.0 nM 7.38
CHEMBL4523041 IC50 = 50.0 nM 7.30
CHEMBL4591330 IC50 = 52.0 nM 7.28
CHEMBL4438387 IC50 = 53.0 nM 7.28
CHEMBL4451381 IC50 = 56.0 nM 7.25
CHEMBL4470513 IC50 = 62.0 nM 7.21
CHEMBL4474529 IC50 = 66.0 nM 7.18
CHEMBL4443351 IC50 = 73.0 nM 7.14
CHEMBL4471087 IC50 = 80.0 nM 7.10
CHEMBL4550097 IC50 = 82.0 nM 7.09
CHEMBL4544397 IC50 = 217.0 nM 6.66
CHEMBL4435018 IC50 = 294.0 nM 6.53
CHEMBL4530061 IC50 = 449.0 nM 6.35
CHEMBL4475159 IC50 = 657.0 nM 6.18