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Assay Detail

CHEMBL4429655

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]-orexin A from human OX1 receptor expressed in CHO cell membranes after 90 mins by scintillation counting
23
Total Activities
12
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Orexin/Hypocretin receptor type 1 (CHEMBL5113)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Pharmacophore Model To Discover OX1 and OX2 Orexin Receptor Ligands.
Turku A, Borrel A, Leino TO, Karhu L, Kukkonen JP, Xhaard H.
J Med Chem (2016) 59 : 8263 -8275
PubMed 27546834 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 12 5.66 7.85
IC50 11 5.19 5.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL291536 1 7.85
CHEMBL4471376 2 6.10
CHEMBL4548914 2 5.96
CHEMBL4578783 2 5.92
CHEMBL3798017 (R)-Carvedilol 2 5.89
CHEMBL4544095 2 5.57
CHEMBL4531556 2 5.20
CHEMBL1481561 2 5.17
CHEMBL4445194 2 5.12
CHEMBL4473709 2 4.92
CHEMBL4475736 2 4.51
CHEMBL4472213 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL291536 Ki = 14.0 nM 7.85
CHEMBL4471376 Ki = 800.0 nM 6.10
CHEMBL4548914 Ki = 1100.0 nM 5.96
CHEMBL4578783 Ki = 1200.0 nM 5.92
CHEMBL3798017 (R)-Carvedilol Ki = 1300.0 nM 5.89
CHEMBL4471376 IC50 = 1400.0 nM 5.85
CHEMBL4548914 IC50 = 1900.0 nM 5.72
CHEMBL4578783 IC50 = 2200.0 nM 5.66
CHEMBL3798017 (R)-Carvedilol IC50 = 2300.0 nM 5.64
CHEMBL4544095 Ki = 2700.0 nM 5.57
CHEMBL4544095 IC50 = 4800.0 nM 5.32
CHEMBL4531556 Ki = 6300.0 nM 5.20
CHEMBL1481561 Ki = 6700.0 nM 5.17
CHEMBL4445194 Ki = 7600.0 nM 5.12
CHEMBL4531556 IC50 = 11100.0 nM 4.96
CHEMBL1481561 IC50 = 11700.0 nM 4.93
CHEMBL4473709 Ki = 12000.0 nM 4.92
CHEMBL4445194 IC50 = 13300.0 nM 4.88
CHEMBL4473709 IC50 = 20900.0 nM 4.68
CHEMBL4475736 Ki = 30700.0 nM 4.51
CHEMBL4475736 IC50 = 53700.0 nM 4.27
CHEMBL4472213 Ki - -
CHEMBL4472213 IC50 - -