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Assay Detail

CHEMBL4432408

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HCV genotype 1b J4 NS5B RNA dependent RNA polymerase
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Hepatitis C virus isolate HC-J4
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Genome polyprotein (CHEMBL3638332)
Type SINGLE PROTEIN
Organism Hepatitis C virus genotype 1b (strain HC-J4) (HCV)

Publication

Synthesis of potent and broad genotypically active NS5B HCV non-nucleoside inhibitors binding to the thumb domain allosteric site 2 of the viral polymerase.
Pierra Rouvière C, Amador A, Badaroux E, Convard T, Da Costa D, Dukhan D, Griffe L, Griffon JF, LaColla M, Leroy F, Liuzzi M, Loi AG, McCarville J, Mascia V, Milhau J, Onidi L, Paparin JL, Rahali R, Sais E, Seifer M, Surleraux D, Standring D, Dousson C.
Bioorg Med Chem Lett (2016) 26 : 4536 -4541
PubMed 27520942 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 28 7.09 8.29

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4527971 1 8.29
CHEMBL4463284 1 8.27
CHEMBL4464159 1 8.25
CHEMBL4451988 1 8.11
CHEMBL4567705 1 8.04
CHEMBL4522572 1 7.79
CHEMBL4464724 1 7.68
CHEMBL4441399 1 7.58
CHEMBL561360 1 7.46
CHEMBL4563132 1 7.34
CHEMBL4578396 1 7.23
CHEMBL4459000 1 7.09
CHEMBL4575406 1 6.93
CHEMBL4584994 1 6.87
CHEMBL4567123 1 6.83
CHEMBL4434914 1 6.81
CHEMBL4463960 1 6.77
CHEMBL4448379 1 6.72
CHEMBL4529821 1 6.65
CHEMBL4465164 1 6.53
CHEMBL4474860 1 6.53
CHEMBL4454342 1 6.50
CHEMBL4541419 1 6.37
CHEMBL4533843 1 6.29
CHEMBL4573414 1 5.95
CHEMBL4445547 1 5.43
CHEMBL4557948 1 -
CHEMBL4552559 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4527971 IC50 = 5.1 nM 8.29
CHEMBL4463284 IC50 = 5.4 nM 8.27
CHEMBL4464159 IC50 = 5.6 nM 8.25
CHEMBL4451988 IC50 = 7.8 nM 8.11
CHEMBL4567705 IC50 = 9.2 nM 8.04
CHEMBL4522572 IC50 = 16.3 nM 7.79
CHEMBL4464724 IC50 = 21.0 nM 7.68
CHEMBL4441399 IC50 = 26.0 nM 7.58
CHEMBL561360 IC50 = 35.0 nM 7.46
CHEMBL4563132 IC50 = 45.8 nM 7.34
CHEMBL4578396 IC50 = 59.0 nM 7.23
CHEMBL4459000 IC50 = 81.0 nM 7.09
CHEMBL4575406 IC50 = 118.0 nM 6.93
CHEMBL4584994 IC50 = 135.0 nM 6.87
CHEMBL4567123 IC50 = 147.0 nM 6.83
CHEMBL4434914 IC50 = 154.0 nM 6.81
CHEMBL4463960 IC50 = 170.0 nM 6.77
CHEMBL4448379 IC50 = 189.0 nM 6.72
CHEMBL4529821 IC50 = 223.0 nM 6.65
CHEMBL4465164 IC50 = 298.0 nM 6.53
CHEMBL4474860 IC50 = 298.0 nM 6.53
CHEMBL4454342 IC50 = 314.0 nM 6.50
CHEMBL4541419 IC50 = 425.0 nM 6.37
CHEMBL4533843 IC50 = 517.0 nM 6.29
CHEMBL4573414 IC50 = 1126.0 nM 5.95
CHEMBL4445547 IC50 = 3740.0 nM 5.43
CHEMBL4557948 IC50 > 10000000.0 nM -
CHEMBL4552559 IC50 > 10000.0 nM -