Assay Detail
Binding
CHEMBL4432415
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Inhibition of Escherichia coli DNA polymerase 1 Klenow fragment preincubated for 15 mins followed by addition of DNA substrate for 60 mins in presence of dNTPS by picoGreen reagent based assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Escherichia coli |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
A fluorescence polarization based assay for the identification and characterization of polymerase inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 1 | 4.16 | 4.16 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1383 | DDA-TRIPHOSPHATE | -1.0 | 1 | 4.16 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1383 | DDA-TRIPHOSPHATE | IC50 | = | 69000.0 | nM | 4.16 |