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Assay Detail

CHEMBL4432466

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of VMAT2 in rat striatal homogenate assessed as reduction in [3H]DA uptake after 8 mins by liquid scintillation spectrometric analysis
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Striatum
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Synaptic vesicular amine transporter (CHEMBL4828)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Synthesis and in vitro evaluation of water-soluble 1,4-diphenethylpiperazine analogs as novel inhibitors of the vesicular monoamine transporter-2.
Nickell JR, Culver JP, Janganati V, Zheng G, Dwoskin LP, Crooks PA.
Bioorg Med Chem Lett (2016) 26 : 4441 -4445
PubMed 27524311 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 11 7.12 7.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4443407 1 7.46
CHEMBL4519939 1 7.43
CHEMBL4442303 1 7.32
CHEMBL4518586 1 7.24
CHEMBL4531091 1 7.22
CHEMBL4444122 1 7.20
CHEMBL4444692 1 7.06
CHEMBL4452875 1 7.01
CHEMBL4532351 1 7.00
CHEMBL4473454 1 6.96
CHEMBL4444796 1 6.39

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4443407 Ki = 35.0 nM 7.46
CHEMBL4519939 Ki = 37.0 nM 7.43
CHEMBL4442303 Ki = 48.0 nM 7.32
CHEMBL4518586 Ki = 58.0 nM 7.24
CHEMBL4531091 Ki = 60.0 nM 7.22
CHEMBL4444122 Ki = 63.0 nM 7.20
CHEMBL4444692 Ki = 88.0 nM 7.06
CHEMBL4452875 Ki = 98.0 nM 7.01
CHEMBL4532351 Ki = 100.0 nM 7.00
CHEMBL4473454 Ki = 110.0 nM 6.96
CHEMBL4444796 Ki = 410.0 nM 6.39