Assay Detail
Binding
CHEMBL4433811
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Transactivation of GAL4-tagged human PPARalpha LBD expressed in human HepG2 cells at 100 nM to 100 uM incubated for 20 to 22 hrs by luciferase reporter gene assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HepG2 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Peroxisome proliferator-activated receptor alpha (CHEMBL239) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Novel Phenyldiazenyl Fibrate Analogues as PPAR α/γ/δ Pan-Agonists for the Amelioration of Metabolic Syndrome.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 14 | 5.79 | 6.60 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4449760 | — | — | 1 | 6.60 |
| CHEMBL4471853 | — | — | 1 | 6.42 |
| CHEMBL4461949 | — | — | 1 | 6.29 |
| CHEMBL4453610 | — | — | 1 | 6.09 |
| CHEMBL4557173 | — | — | 1 | 5.85 |
| CHEMBL4533822 | — | — | 1 | 5.72 |
| CHEMBL2204696 | — | — | 1 | 5.68 |
| CHEMBL4438226 | — | — | 1 | 5.66 |
| CHEMBL4445737 | — | — | 1 | 5.64 |
| CHEMBL4592451 | — | — | 1 | 5.62 |
| CHEMBL4461490 | — | — | 1 | 5.58 |
| CHEMBL683 | CLOFIBRIC ACID | 2.0 | 1 | 4.30 |
| CHEMBL4444322 | — | — | 1 | - |
| CHEMBL4580572 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4449760 | — | EC50 | = | 250.0 | nM | 6.60 |
| CHEMBL4471853 | — | EC50 | = | 380.0 | nM | 6.42 |
| CHEMBL4461949 | — | EC50 | = | 510.0 | nM | 6.29 |
| CHEMBL4453610 | — | EC50 | = | 810.0 | nM | 6.09 |
| CHEMBL4557173 | — | EC50 | = | 1400.0 | nM | 5.85 |
| CHEMBL4533822 | — | EC50 | = | 1900.0 | nM | 5.72 |
| CHEMBL2204696 | — | EC50 | = | 2100.0 | nM | 5.68 |
| CHEMBL4438226 | — | EC50 | = | 2200.0 | nM | 5.66 |
| CHEMBL4445737 | — | EC50 | = | 2300.0 | nM | 5.64 |
| CHEMBL4592451 | — | EC50 | = | 2400.0 | nM | 5.62 |
| CHEMBL4461490 | — | EC50 | = | 2600.0 | nM | 5.58 |
| CHEMBL683 | CLOFIBRIC ACID | EC50 | = | 50000.0 | nM | 4.30 |
| CHEMBL4444322 | — | EC50 | - | — | - | |
| CHEMBL4580572 | — | EC50 | - | — | - |