Assay Detail
Binding
CHEMBL4434258
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of mTOR in mouse TSC1-/- MEF cells assessed as inhibition of S6 Ser240/244 phosphorylation incubated for 2 hrs by fluorescence based assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Cell Type | MEF |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Serine/threonine-protein kinase mTOR (CHEMBL1255165) ↗| Type | SINGLE PROTEIN |
| Organism | Mus musculus |
Publication
Rapalogs Potential as Practical Alternatives to Rapamycin.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 6 | 9.06 | 10.30 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL413 | SIROLIMUS | 4.0 | 1 | 10.30 |
| CHEMBL4529771 | — | — | 1 | 9.77 |
| CHEMBL4591064 | — | — | 1 | 9.56 |
| CHEMBL4465976 | — | — | 1 | 9.43 |
| CHEMBL4580738 | — | — | 1 | 8.49 |
| CHEMBL4563398 | — | — | 1 | 6.82 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL413 | SIROLIMUS | IC50 | = | 0.05 | nM | 10.30 |
| CHEMBL4529771 | — | IC50 | = | 0.17 | nM | 9.77 |
| CHEMBL4591064 | — | IC50 | = | 0.274 | nM | 9.56 |
| CHEMBL4465976 | — | IC50 | = | 0.374 | nM | 9.43 |
| CHEMBL4580738 | — | IC50 | = | 3.2 | nM | 8.49 |
| CHEMBL4563398 | — | IC50 | = | 153.0 | nM | 6.82 |