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Assay Detail

CHEMBL4434264

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human GlyT2 expressed in porcine aorta epithelial cells assessed as reduction in [3H]-Glycine uptake at pH 7.5 by scintillation spectroscopy
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium- and chloride-dependent glycine transporter 2 (CHEMBL3060)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of GlyT2 Inhibitors Using Structure-Based Pharmacophore Screening and Selectivity Studies by FEP+ Calculations.
Fratev F, Miranda-Arango M, Lopez AB, Padilla E, Sirimulla S.
ACS Med Chem Lett (2019) 10 : 904 -910
PubMed 31223446 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 6.08 7.58

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL475562 1 7.58
CHEMBL4439878 1 6.32
CHEMBL4452842 1 6.29
CHEMBL180966 1 5.75
CHEMBL4566424 1 4.48

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL475562 IC50 = 26.0 nM 7.58
CHEMBL4439878 IC50 = 478.0 nM 6.32
CHEMBL4452842 IC50 = 518.0 nM 6.29
CHEMBL180966 IC50 = 1800.0 nM 5.75
CHEMBL4566424 IC50 = 33200.0 nM 4.48