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Assay Detail

CHEMBL4434437

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NRF2 in human MDA-MB-231 cells harboring ARE-GFP-Luc assessed as reduction in ARE-luciferase activity after 16 hrs by firefly luciferase reporter gene assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type MDA-MB-231
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nuclear factor erythroid 2-related factor 2 (CHEMBL1075094)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Homoharringtonine stabilizes secondary structure of guanine-rich sequence existing in the 5'-untranslated region of Nrf2.
Kang JS, Lee J, Nam LB, Yoo OK, Pham KT, Duong TH, Keum YS.
Bioorg Med Chem Lett (2019) 29 : 2189 -2196
PubMed 31270017 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 7.54 7.54

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL459546 1 7.54

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL459546 IC50 = 29.19 nM 7.54