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Assay Detail

CHEMBL4478423

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ABCB1 in human A2780/ADR cells assessed as reduction in calcein AM efflux pre-incubated for 30 mins before calcein AM addition and measured for 60 mins by microplate reader analysis relative to control
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

ATP-dependent translocase ABCB1 (CHEMBL4302)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and Investigation of Tetrahydro-β-carboline Derivatives as Inhibitors of the Breast Cancer Resistance Protein (ABCG2).
Spindler A, Stefan K, Wiese M.
J Med Chem (2016) 59 : 6121 -6135
PubMed 27280693 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 5.07 5.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL160 CYCLOSPORINE 4.0 1 5.96
CHEMBL4578134 1 5.54
CHEMBL4592538 1 5.47
CHEMBL4542075 1 5.21
CHEMBL4549558 1 5.18
CHEMBL4550890 1 5.13
CHEMBL4576040 1 5.13
CHEMBL4581843 1 5.10
CHEMBL4575544 1 5.08
CHEMBL4521174 1 5.06
CHEMBL4549093 1 5.04
CHEMBL4559337 1 5.02
CHEMBL4586159 1 4.95
CHEMBL4516431 1 4.92
CHEMBL4593846 1 4.89
CHEMBL4555019 1 4.87
CHEMBL4568684 1 4.84
CHEMBL4571543 1 4.72
CHEMBL4549202 1 4.65
CHEMBL4563533 1 4.63

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL160 CYCLOSPORINE IC50 = 1090.0 nM 5.96
CHEMBL4578134 IC50 = 2860.0 nM 5.54
CHEMBL4592538 IC50 = 3380.0 nM 5.47
CHEMBL4542075 IC50 = 6220.0 nM 5.21
CHEMBL4549558 IC50 = 6550.0 nM 5.18
CHEMBL4550890 IC50 = 7330.0 nM 5.13
CHEMBL4576040 IC50 = 7400.0 nM 5.13
CHEMBL4581843 IC50 = 7940.0 nM 5.10
CHEMBL4575544 IC50 = 8240.0 nM 5.08
CHEMBL4521174 IC50 = 8760.0 nM 5.06
CHEMBL4549093 IC50 = 9230.0 nM 5.04
CHEMBL4559337 IC50 = 9550.0 nM 5.02
CHEMBL4586159 IC50 = 11300.0 nM 4.95
CHEMBL4516431 IC50 = 12000.0 nM 4.92
CHEMBL4593846 IC50 = 12900.0 nM 4.89
CHEMBL4555019 IC50 = 13400.0 nM 4.87
CHEMBL4568684 IC50 = 14300.0 nM 4.84
CHEMBL4571543 IC50 = 19200.0 nM 4.72
CHEMBL4549202 IC50 = 22500.0 nM 4.65
CHEMBL4563533 IC50 = 23400.0 nM 4.63