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Assay Detail

CHEMBL4479676

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full-length recombinant human GST-tagged MARK3 expressed in baculovirus expression system using biotinylated-Cdc25C peptide substrate measured after 2 hrs by HTRF assay
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

MAP/microtubule affinity-regulating kinase 3 (CHEMBL5600)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimization of microtubule affinity regulating kinase (MARK) inhibitors with improved physical properties.
Sloman DL, Noucti N, Altman MD, Chen D, Mislak AC, Szewczak A, Hayashi M, Warren L, Dellovade T, Wu Z, Marcus J, Walker D, Su HP, Edavettal SC, Munshi S, Hutton M, Nuthall H, Stanton MG.
Bioorg Med Chem Lett (2016) 26 : 4362 -4366
PubMed 27491711 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 7.93 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4569508 1 9.00
CHEMBL4532781 1 9.00
CHEMBL4550486 1 8.89
CHEMBL4528550 1 8.70
CHEMBL4552799 1 8.62
CHEMBL4562217 1 8.52
CHEMBL4552628 1 8.30
CHEMBL4593028 1 8.30
CHEMBL4550702 1 8.22
CHEMBL4579339 1 8.05
CHEMBL4571737 1 8.05
CHEMBL4543195 1 8.00
CHEMBL4569165 1 8.00
CHEMBL4547786 1 7.89
CHEMBL4557345 1 7.82
CHEMBL4578265 1 7.82
CHEMBL4589674 1 7.66
CHEMBL4544609 1 7.62
CHEMBL4526375 1 7.42
CHEMBL4588200 1 7.37
CHEMBL4550262 1 7.16
CHEMBL4541288 1 7.13
CHEMBL4514812 1 7.00
CHEMBL4556844 1 6.89
CHEMBL4561857 1 6.89
CHEMBL4568087 1 -
CHEMBL4565845 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4569508 IC50 = 1.0 nM 9.00
CHEMBL4532781 IC50 = 1.0 nM 9.00
CHEMBL4550486 IC50 = 1.3 nM 8.89
CHEMBL4528550 IC50 = 2.0 nM 8.70
CHEMBL4552799 IC50 = 2.4 nM 8.62
CHEMBL4562217 IC50 = 3.0 nM 8.52
CHEMBL4552628 IC50 = 5.0 nM 8.30
CHEMBL4593028 IC50 = 5.0 nM 8.30
CHEMBL4550702 IC50 = 6.0 nM 8.22
CHEMBL4579339 IC50 = 9.0 nM 8.05
CHEMBL4571737 IC50 = 9.0 nM 8.05
CHEMBL4543195 IC50 = 10.0 nM 8.00
CHEMBL4569165 IC50 = 10.0 nM 8.00
CHEMBL4547786 IC50 = 13.0 nM 7.89
CHEMBL4557345 IC50 = 15.0 nM 7.82
CHEMBL4578265 IC50 = 15.0 nM 7.82
CHEMBL4589674 IC50 = 22.0 nM 7.66
CHEMBL4544609 IC50 = 24.0 nM 7.62
CHEMBL4526375 IC50 = 38.0 nM 7.42
CHEMBL4588200 IC50 = 43.0 nM 7.37
CHEMBL4550262 IC50 = 70.0 nM 7.16
CHEMBL4541288 IC50 = 74.0 nM 7.13
CHEMBL4514812 IC50 = 99.0 nM 7.00
CHEMBL4556844 IC50 = 129.0 nM 6.89
CHEMBL4561857 IC50 = 130.0 nM 6.89
CHEMBL4568087 IC50 <= 0.5 nM -
CHEMBL4565845 IC50 < 0.25 nM -