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Assay Detail

CHEMBL4480332

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of calpain (unknown origin) using calpain substrate preincubated for 20 mins followed by substrate addition by fluorescence plate reader analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Calpain1/2 (CHEMBL3038466)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

Discovery of Fluoromethylketone-Based Peptidomimetics as Covalent ATG4B (Autophagin-1) Inhibitors.
Qiu Z, Kuhn B, Aebi J, Lin X, Ding H, Zhou Z, Xu Z, Xu D, Han L, Liu C, Qiu H, Zhang Y, Haap W, Riemer C, Stahl M, Qin N, Shen HC, Tang G.
ACS Med Chem Lett (2016) 7 : 802 -806
PubMed 27563406 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 6.49 7.02

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4520267 1 7.02
CHEMBL2402203 1 6.22
CHEMBL4590201 1 6.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4520267 IC50 = 96.0 nM 7.02
CHEMBL2402203 IC50 = 600.0 nM 6.22
CHEMBL4590201 IC50 = 600.0 nM 6.22