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Assay Detail

CHEMBL4480597

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Binding
Antagonist activity at histamine H1 receptor in guinea pig ileum preincubated for 10 mins followed by histamine addition during 30 mins equilibration period with washing every 10 mins
9
Total Activities
9
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Cavia porcellus
Tissue Ileum
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H1 receptor (CHEMBL3943)
Type SINGLE PROTEIN
Organism Cavia porcellus

Publication

Design, synthesis, and <i>in vitro</i> and <i>in vivo</i> characterization of 1-{4-[4-(substituted)piperazin-1-yl]butyl}guanidines and their piperidine analogues as histamine H<sub>3</sub> receptor antagonists.
Staszewski M, Stasiak A, Karcz T, McNaught Flores D, Fogel WA, Kieć-Kononowicz K, Leurs R, Walczyński K.
Medchemcomm (2019) 10 : 234 -251
PubMed 30881612 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 8 7.32 9.18
pA2 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL511 PYRILAMINE 4.0 1 9.18
CHEMBL4514561 1 7.22
CHEMBL4546027 1 7.19
CHEMBL4579190 1 7.18
CHEMBL4482871 1 7.10
CHEMBL4517581 1 6.97
CHEMBL4585110 1 6.90
CHEMBL4550675 1 6.80
CHEMBL4591503 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL511 PYRILAMINE Kd = 0.6607 nM 9.18
CHEMBL4514561 Kd = 60.26 nM 7.22
CHEMBL4546027 Kd = 64.57 nM 7.19
CHEMBL4579190 Kd = 66.07 nM 7.18
CHEMBL4482871 Kd = 79.43 nM 7.10
CHEMBL4517581 Kd = 107.15 nM 6.97
CHEMBL4585110 Kd = 125.89 nM 6.90
CHEMBL4550675 Kd = 158.49 nM 6.80
CHEMBL4591503 pA2 - -