Assay Detail
Binding
CHEMBL4480649
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of N-terminal His6-tagged truncated PHD2 (181 to 426 residues) (unknown origin) expressed in Escherichia coli BL21 DE3 using H1F1-alpha NODD as substrate preincubated for 15 mins followed by substrate addition and measured after 15 mins by mass spectrometry
20
Total Activities
20
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Studies on spiro[4.5]decanone prolyl hydroxylase domain inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 11 | 6.91 | 7.31 |
| Inhibition | 9 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4536280 | — | — | 1 | 7.31 |
| CHEMBL4535543 | — | — | 1 | 7.26 |
| CHEMBL4482885 | — | — | 1 | 7.20 |
| CHEMBL4557378 | — | — | 1 | 7.16 |
| CHEMBL4526672 | — | — | 1 | 7.03 |
| CHEMBL2043005 | — | — | 1 | 6.90 |
| CHEMBL2042999 | — | — | 1 | 6.37 |
| CHEMBL4514222 | — | — | 1 | 6.04 |
| CHEMBL4537110 | — | — | 1 | - |
| CHEMBL4525775 | — | — | 1 | - |
| CHEMBL4563706 | — | — | 1 | - |
| CHEMBL4557919 | — | — | 1 | - |
| CHEMBL4574692 | — | — | 1 | - |
| CHEMBL4555144 | — | — | 1 | - |
| CHEMBL4531046 | — | — | 1 | - |
| CHEMBL4569324 | — | — | 1 | - |
| CHEMBL4589011 | — | — | 1 | - |
| CHEMBL4561626 | — | — | 1 | - |
| CHEMBL4573683 | — | — | 1 | - |
| CHEMBL4579945 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4536280 | — | IC50 | = | 49.0 | nM | 7.31 |
| CHEMBL4535543 | — | IC50 | = | 55.0 | nM | 7.26 |
| CHEMBL4482885 | — | IC50 | = | 63.0 | nM | 7.20 |
| CHEMBL4557378 | — | IC50 | = | 70.0 | nM | 7.16 |
| CHEMBL4526672 | — | IC50 | = | 94.0 | nM | 7.03 |
| CHEMBL2043005 | — | IC50 | = | 127.0 | nM | 6.90 |
| CHEMBL2042999 | — | IC50 | = | 427.0 | nM | 6.37 |
| CHEMBL4514222 | — | IC50 | = | 905.0 | nM | 6.04 |
| CHEMBL4537110 | — | Inhibition | - | % | - | |
| CHEMBL4525775 | — | Inhibition | - | % | - | |
| CHEMBL4563706 | — | Inhibition | - | % | - | |
| CHEMBL4557919 | — | Inhibition | - | % | - | |
| CHEMBL4574692 | — | Inhibition | - | % | - | |
| CHEMBL4555144 | — | Inhibition | - | % | - | |
| CHEMBL4531046 | — | Inhibition | - | % | - | |
| CHEMBL4569324 | — | Inhibition | - | % | - | |
| CHEMBL4589011 | — | Inhibition | - | % | - | |
| CHEMBL4561626 | — | IC50 | > | 25000.0 | nM | - |
| CHEMBL4573683 | — | IC50 | > | 25000.0 | nM | - |
| CHEMBL4579945 | — | IC50 | > | 25000.0 | nM | - |