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Assay Detail

CHEMBL4482009

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human JAK2 using TK-substrate-biotin preincubated for 5 mins followed by substrate addition and further incubated for 30 mins and subsequently measured after 1 hr by HTRF assay
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK2 (CHEMBL2971)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-based design and synthesis of pyrimidine-4,6-diamine derivatives as Janus kinase 3 inhibitors.
Yu RN, Chen CJ, Shu L, Yin Y, Wang ZJ, Zhang TT, Zhang DY.
Bioorg Med Chem (2019) 27 : 1646 -1657
PubMed 30853331 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 31 6.31 9.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL221959 TOFACITINIB 4.0 1 9.30
CHEMBL4589415 1 6.55
CHEMBL4581532 1 6.46
CHEMBL4516549 1 6.46
CHEMBL4591192 1 6.44
CHEMBL4524497 1 6.30
CHEMBL4566849 1 6.04
CHEMBL3601722 1 6.03
CHEMBL4546009 1 5.99
CHEMBL4573218 1 5.96
CHEMBL4518786 1 5.84
CHEMBL4574614 1 5.69
CHEMBL4541547 1 5.68
CHEMBL4525246 1 5.55
CHEMBL4539137 1 -
CHEMBL4526018 1 -
CHEMBL4572373 1 -
CHEMBL4593058 1 -
CHEMBL4577029 1 -
CHEMBL4566777 1 -
CHEMBL4537782 1 -
CHEMBL4588496 1 -
CHEMBL4521305 1 -
CHEMBL4555932 1 -
CHEMBL4587735 1 -
CHEMBL4560222 1 -
CHEMBL4528217 1 -
CHEMBL4552985 1 -
CHEMBL4563864 1 -
CHEMBL4571470 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL221959 TOFACITINIB IC50 = 0.5 nM 9.30
CHEMBL4589415 IC50 = 280.0 nM 6.55
CHEMBL4581532 IC50 = 350.0 nM 6.46
CHEMBL4516549 IC50 = 345.0 nM 6.46
CHEMBL4591192 IC50 = 366.0 nM 6.44
CHEMBL4524497 IC50 = 503.0 nM 6.30
CHEMBL4566849 IC50 = 916.0 nM 6.04
CHEMBL3601722 IC50 = 945.0 nM 6.03
CHEMBL4546009 IC50 = 1015.0 nM 5.99
CHEMBL4573218 IC50 = 1096.0 nM 5.96
CHEMBL4518786 IC50 = 1444.0 nM 5.84
CHEMBL4574614 IC50 = 2043.0 nM 5.69
CHEMBL4541547 IC50 = 2098.0 nM 5.68
CHEMBL4525246 IC50 = 2790.0 nM 5.55
CHEMBL4556768 IC50 > 3000.0 nM -
CHEMBL4539137 IC50 > 3000.0 nM -
CHEMBL4526018 IC50 > 3000.0 nM -
CHEMBL4572373 IC50 > 3000.0 nM -
CHEMBL4593058 IC50 - -
CHEMBL4577029 IC50 > 3000.0 nM -
CHEMBL4566777 IC50 - -
CHEMBL4537782 IC50 - -
CHEMBL4588496 IC50 - -
CHEMBL4521305 IC50 - -
CHEMBL4555932 IC50 - -
CHEMBL4587735 IC50 > 3000.0 nM -
CHEMBL4560222 IC50 - -
CHEMBL4528217 IC50 - -
CHEMBL4552985 IC50 - -
CHEMBL4563864 IC50 - -
CHEMBL4571470 IC50 - -