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Assay Detail

CHEMBL4482622

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of RAD52 (unknown origin) assessed as reduction in RAD52-ssDNA interaction using Cy3-dT30-Cy5 ssDNA in presence of replication protein A by FRET assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

DNA repair protein RAD52 homolog (CHEMBL2362978)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

HTS Assay for Identifying Small Molecule Inhibitors of RAD52 and Uses of Identified Small Molecule Inhibitors for Treatment and Prevention of BRCA-Deficient Malignancies
(2018)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 5.97 6.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL449392 CORILAGIN 1 6.30
CHEMBL47386 EPIGALLOCATECHIN 1 5.80
CHEMBL297453 EPIGALOCATECHIN GALLATE 3.0 1 5.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL449392 CORILAGIN IC50 = 500.0 nM 6.30
CHEMBL47386 EPIGALLOCATECHIN IC50 = 1600.0 nM 5.80
CHEMBL297453 EPIGALOCATECHIN GALLATE IC50 = 1600.0 nM 5.80