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Assay Detail

CHEMBL4603183

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Aggregation-based inhibition of human recombinant AKR1B1 assessed as D,L-glyceraldehyde reduction incubated in sodium phosphate buffer at pH 6.2 in presence of NADPH and 0.05% Triton X-100 by detergent-addition assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Aldo-keto reductase family 1 member B1 (CHEMBL1900)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Early identification of promiscuous attributes of aldose reductase inhibitors using a DMSO-perturbation assay.
Tomohara K, Hasegawa N, Adachi I, Horino Y, Nose T.
Bioorg Med Chem Lett (2020) 30 : 126815 -126815
PubMed 31744675 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 4.23 4.32

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1908021 1 4.32
CHEMBL4640749 1 4.14
CHEMBL1869748 1 -
CHEMBL289046 1 -
CHEMBL4635409 1 -
CHEMBL4648564 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1908021 IC50 = 47900.0 nM 4.32
CHEMBL4640749 IC50 = 71900.0 nM 4.14
CHEMBL1869748 IC50 = 319000.0 nM -
CHEMBL289046 IC50 = 114000.0 nM -
CHEMBL4635409 IC50 = 453000.0 nM -
CHEMBL4648564 IC50 = 433000.0 nM -