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Assay Detail

CHEMBL4603185

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant AKR1B1 assessed as D,L-glyceraldehyde reduction pretreated with 0.3M DMSO followed by compound addition by DMSO-perturbation assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Aldo-keto reductase family 1 member B1 (CHEMBL1900)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Early identification of promiscuous attributes of aldose reductase inhibitors using a DMSO-perturbation assay.
Tomohara K, Hasegawa N, Adachi I, Horino Y, Nose T.
Bioorg Med Chem Lett (2020) 30 : 126815 -126815
PubMed 31744675 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 5.79 7.42

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL10372 ZOPOLRESTAT 2.0 1 7.42
CHEMBL56337 EPALRESTAT 4.0 1 6.96
CHEMBL266497 SORBINIL 3.0 1 6.01
CHEMBL1908021 1 4.39
CHEMBL4640749 1 4.18

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL10372 ZOPOLRESTAT IC50 = 37.8 nM 7.42
CHEMBL56337 EPALRESTAT IC50 = 110.0 nM 6.96
CHEMBL266497 SORBINIL IC50 = 971.0 nM 6.01
CHEMBL1908021 IC50 = 40600.0 nM 4.39
CHEMBL4640749 IC50 = 65600.0 nM 4.18