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Assay Detail

CHEMBL4604199

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human placental SAH hydrolase expressed in Escherichia coli JM109 using SAH as substrate preincubated for 10 mins followed by SAH addition and measured after 20 mins by DNTB reagent-based spectrophotometric method
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosylhomocysteinase (CHEMBL2664)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of 6'-β-fluoro-homoaristeromycin as a potent inhibitor of chikungunya virus replication.
Shin YS, Jarhad DB, Jang MH, Kovacikova K, Kim G, Yoon JS, Kim HR, Hyun YE, Tipnis AS, Chang TS, van Hemert MJ, Jeong LS.
Eur J Med Chem (2020) 187 : 111956 -111956
PubMed 31841728 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 6.08 6.44

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4638533 1 6.44
CHEMBL4635734 1 6.43
CHEMBL2115462 1 6.43
CHEMBL4544781 1 5.97
CHEMBL4644901 1 5.69
CHEMBL4637324 1 5.52
CHEMBL4640283 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4638533 IC50 = 360.0 nM 6.44
CHEMBL4635734 IC50 = 370.0 nM 6.43
CHEMBL2115462 IC50 = 370.0 nM 6.43
CHEMBL4544781 IC50 = 1060.0 nM 5.97
CHEMBL4644901 IC50 = 2030.0 nM 5.69
CHEMBL4637324 IC50 = 3050.0 nM 5.52
CHEMBL4640283 IC50 > 100000.0 nM -