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Assay Detail

CHEMBL4604968

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human His/GST-tagged GSK3beta (2 to 433 residues) expressed in baculovirus infected Sf9 cells using Ulight-glycogen synthase peptide as substrate incubated for 30 mins by TR-FRET LANCE method
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glycogen synthase kinase-3 beta (CHEMBL262)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Selective DYRK1A Inhibitor for the Treatment of Type 1 Diabetes: Discovery of 6-Azaindole Derivative GNF2133.
Liu YA, Jin Q, Zou Y, Ding Q, Yan S, Wang Z, Hao X, Nguyen B, Zhang X, Pan J, Mo T, Jacobsen K, Lam T, Wu TY, Petrassi HM, Bursulaya B, DiDonato M, Gordon WP, Liu B, Baaten J, Hill R, Nguyen-Tran V, Qiu M, Zhang YQ, Kamireddy A, Espinola S, Deaton L, Ha S, Harb G, Jia Y, Li J, Shen W, Schumacher AM, Colman K, Glynne R, Pan S, McNamara P, Laffitte B, Meeusen S, Molteni V, Loren J.
J Med Chem (2020) 63 : 2958 -2973
PubMed 32077280 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 5.06 6.20

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4648047 1 6.20
CHEMBL4640465 1 5.71
CHEMBL4641631 1 5.25
CHEMBL4091320 1 4.53
CHEMBL4640598 1 4.35
CHEMBL4641672 1 4.33
CHEMBL4634969 1 -
CHEMBL4648945 1 -
CHEMBL4644898 1 -
CHEMBL4638460 1 -
CHEMBL4648742 1 -
CHEMBL4645474 1 -
CHEMBL4649473 1 -
CHEMBL4641839 1 -
CHEMBL4640031 1 -
CHEMBL4636064 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4648047 IC50 = 630.0 nM 6.20
CHEMBL4640465 IC50 = 1950.0 nM 5.71
CHEMBL4641631 IC50 = 5640.0 nM 5.25
CHEMBL4091320 IC50 = 29600.0 nM 4.53
CHEMBL4640598 IC50 = 44480.0 nM 4.35
CHEMBL4641672 IC50 = 46500.0 nM 4.33
CHEMBL4634969 IC50 > 8300.0 nM -
CHEMBL4648945 IC50 > 8300.0 nM -
CHEMBL4644898 IC50 > 28500.0 nM -
CHEMBL4638460 IC50 > 12400.0 nM -
CHEMBL4648742 IC50 > 8300.0 nM -
CHEMBL4645474 IC50 > 50000.0 nM -
CHEMBL4649473 IC50 > 50000.0 nM -
CHEMBL4641839 IC50 > 50000.0 nM -
CHEMBL4640031 IC50 > 50000.0 nM -
CHEMBL4636064 IC50 > 50000.0 nM -