Assay Detail
Binding
CHEMBL4605759
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of heme binding to IDO1 in interferon-gamma-induced human HeLa cells assessed as reduction in N-formylkynurenine production using L-tryptophan as substrate incubated for 48 hrs by fluorescence based assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HeLa |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Strategic Incorporation of Polarity in Heme-Displacing Inhibitors of Indoleamine-2,3-dioxygenase-1 (IDO1).
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 12 | 7.95 | 8.77 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4638223 | — | — | 1 | 8.77 |
| CHEMBL4645108 | — | — | 1 | 8.68 |
| CHEMBL4635112 | — | — | 1 | 8.57 |
| CHEMBL4632601 | — | — | 1 | 8.49 |
| CHEMBL4633874 | — | — | 1 | 8.44 |
| CHEMBL4637907 | — | — | 1 | 8.08 |
| CHEMBL4636709 | — | — | 1 | 8.04 |
| CHEMBL4645220 | — | — | 1 | 7.77 |
| CHEMBL4648885 | — | — | 1 | 7.72 |
| CHEMBL4643572 | — | — | 1 | 7.50 |
| CHEMBL4637454 | — | — | 1 | 5.42 |
| CHEMBL4632911 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4638223 | — | IC50 | = | 1.7 | nM | 8.77 |
| CHEMBL4645108 | — | IC50 | = | 2.1 | nM | 8.68 |
| CHEMBL4635112 | — | IC50 | = | 2.7 | nM | 8.57 |
| CHEMBL4632601 | — | IC50 | = | 3.2 | nM | 8.49 |
| CHEMBL4633874 | — | IC50 | = | 3.6 | nM | 8.44 |
| CHEMBL4637907 | — | IC50 | = | 8.3 | nM | 8.08 |
| CHEMBL4636709 | — | IC50 | = | 9.2 | nM | 8.04 |
| CHEMBL4645220 | — | IC50 | = | 17.0 | nM | 7.77 |
| CHEMBL4648885 | — | IC50 | = | 19.0 | nM | 7.72 |
| CHEMBL4643572 | — | IC50 | = | 32.0 | nM | 7.50 |
| CHEMBL4637454 | — | IC50 | = | 3800.0 | nM | 5.42 |
| CHEMBL4632911 | — | IC50 | > | 10000.0 | nM | - |