Assay Detail
Binding
CHEMBL4605939
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to GST-tagged human EPAC1 CNBD (149 to 318 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins by 8-NBD-cAMP based competitive fluorescence assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Rap guanine nucleotide exchange factor 3 (CHEMBL2029197) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Mechanism of Action of an EPAC1-Selective Competitive Partial Agonist.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 2 | 5.27 | 5.35 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL316966 | CYCLIC ADENOSINE MONOPHOSPHATE | — | 1 | 5.35 |
| CHEMBL4534834 | — | — | 1 | 5.18 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL316966 | CYCLIC ADENOSINE MONOPHOSPHATE | Kd | = | 4500.0 | nM | 5.35 |
| CHEMBL4534834 | — | Kd | = | 6600.0 | nM | 5.18 |