Assay Detail
Functional
CHEMBL4606548
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
UR-DEBa242: A Py-5-Labeled Fluorescent Multipurpose Probe for Investigations on the Histamine H3 and H4 Receptors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 3 | 7.28 | 7.77 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1533310 | HISTAMINE DIHYDROCHLORIDE | 4.0 | 1 | 7.77 |
| CHEMBL4642520 | — | — | 1 | 7.14 |
| CHEMBL260374 | THIOPERAMIDE | — | 1 | 6.92 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1533310 | HISTAMINE DIHYDROCHLORIDE | EC50 | = | 16.98 | nM | 7.77 |
| CHEMBL4642520 | — | EC50 | = | 72.44 | nM | 7.14 |
| CHEMBL260374 | THIOPERAMIDE | EC50 | = | 120.23 | nM | 6.92 |