Assay Detail
Binding
CHEMBL4606571
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay
4
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK-293T |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
UR-DEBa242: A Py-5-Labeled Fluorescent Multipurpose Probe for Investigations on the Histamine H3 and H4 Receptors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 4 | 7.44 | 7.78 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4635634 | — | — | 2 | 7.78 |
| CHEMBL4642520 | — | — | 2 | 7.11 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4635634 | — | Kd | = | 16.5 | nM | 7.78 |
| CHEMBL4635634 | — | Kd | = | 17.78 | nM | 7.75 |
| CHEMBL4642520 | — | Kd | = | 77.62 | nM | 7.11 |
| CHEMBL4642520 | — | Kd | = | 79.5 | nM | 7.10 |