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Assay Detail

CHEMBL4610252

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiviral activity against HCV genotype 1b Con1 expressing wild type NS5B infected in human HuH7 replicon cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene based assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Hepacivirus hominis
Cell Type Huh-7
Confidence 1 — Organism
Curated By Autocuration

Target

Hepatitis C virus (CHEMBL379)
Type ORGANISM
Organism Hepacivirus hominis

Publication

Synthesis and evaluation of 2'-dihalo ribonucleotide prodrugs with activity against hepatitis C virus.
Chris Krueger A, Chen HJ, Randolph JT, Brown BS, Halvorsen GT, Heyman HR, Li T, Marvin CC, Shanley JP, Voight EA, Bow DAJ, Van Handel C, Peterkin V, Carr RA, Stolarik D, Dekhtyar T, Irvin ML, Krishnan P, Henry RF, Wagner R, DeGoey DA.
Bioorg Med Chem (2020) 28 : 115208 -115208
PubMed 31740203 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 3 7.52 7.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4649858 1 7.82
CHEMBL4636436 1 7.52
CHEMBL1259059 SOFOSBUVIR 4.0 1 7.21

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4649858 EC50 = 15.2 nM 7.82
CHEMBL4636436 EC50 = 30.4 nM 7.52
CHEMBL1259059 SOFOSBUVIR EC50 = 62.0 nM 7.21