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Assay Detail

CHEMBL4611124

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PKCbeta in rat RBL2H3 cells assessed as inhibition of mouse anti-DNP IgE-stimulated beta-hexosaminidase release preincubated for 15 mins followed by mouse anti-DNP IgE stimulation and measured after 3 hrs by 4-nitropheny1-2-acetamide-2-deoxy-beta-glucopyranoside substrate based colorimetric method
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type RBL-2H3
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Protein kinase C beta type (CHEMBL3020)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Variegatic acid from the edible mushroom Tylopilus ballouii inhibits TNF-α production and PKCβ1 activity in leukemia cells.
Sugaya K, Ino M, Matsuo N, Onose JI, Abe N.
Bioorg Med Chem Lett (2020) 30 : 126886 -126886
PubMed 31879206 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 4.98 4.98

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4636684 1 4.98

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4636684 IC50 = 10400.0 nM 4.98