Assay Detail
Binding
CHEMBL4611794
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human PDE4D catalytic domain (86 to 413 residues) expressed in Escherichia coli BL21 (DE3) cells using [3H]cAMP as substrate measured after 30 mins by scintillation proximity assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
3',5'-cyclic-AMP phosphodiesterase 4D (CHEMBL288) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Diterpenoids from the Root Bark of <i>Pinus massoniana</i> and Evaluation of Their Phosphodiesterase Type 4D Inhibitory Activity.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 6.12 | 7.32 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL514800 | APREMILAST | 4.0 | 1 | 7.32 |
| CHEMBL4647105 | — | — | 1 | 5.55 |
| CHEMBL3590338 | — | — | 1 | 5.48 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL514800 | APREMILAST | IC50 | = | 47.6 | nM | 7.32 |
| CHEMBL4647105 | — | IC50 | = | 2800.0 | nM | 5.55 |
| CHEMBL3590338 | — | IC50 | = | 3300.0 | nM | 5.48 |