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Assay Detail

CHEMBL4614036

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human DAT transiently expressed in COS7 cells assessed as reduction in [3H]-DA uptake measured after 5 mins by beta-scintillation counting method
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type COS-7
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium-dependent dopamine transporter (CHEMBL238)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Activity Relationships for a Series of (Bis(4-fluorophenyl)methyl)sulfinyl Alkyl Alicyclic Amines at the Dopamine Transporter: Functionalizing the Terminal Nitrogen Affects Affinity, Selectivity, and Metabolic Stability.
Slack RD, Ku TC, Cao J, Giancola JB, Bonifazi A, Loland CJ, Gadiano A, Lam J, Rais R, Slusher BS, Coggiano M, Tanda G, Newman AH.
J Med Chem (2020) 63 : 2343 -2357
PubMed 31661268 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 4 6.95 7.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4639814 1 7.96
CHEMBL4636778 1 7.00
CHEMBL4640372 1 6.57
CHEMBL4634776 1 6.26

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4639814 Ki = 11.1 nM 7.96
CHEMBL4636778 Ki = 100.0 nM 7.00
CHEMBL4640372 Ki = 268.0 nM 6.57
CHEMBL4634776 Ki = 552.0 nM 6.26