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Assay Detail

CHEMBL4615083

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at 5HT2A receptor (unknown origin) expressed in HEK293 cells assessed as inhibition of serotonin hydrochloride-induced calcium flux preincubated for 1 hr followed by serotonin hydrochloride addition by calcium 6 dye based FLIPR assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 2A (CHEMBL224)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, Synthesis, and Biological Evaluation of New Peripheral 5HT2A Antagonists for Nonalcoholic Fatty Liver Disease.
Kim M, Hwang I, Pagire HS, Pagire SH, Choi W, Choi WG, Yoon J, Lee WM, Song JS, Yoo EK, Lee SM, Kim MJ, Bae MA, Kim D, Lee H, Lee EY, Jeon JH, Lee IK, Kim H, Ahn JH.
J Med Chem (2020) 63 : 4171 -4182
PubMed 32285676 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 7.78 9.41

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4635010 1 9.41
CHEMBL4644559 1 9.22
CHEMBL4647697 1 9.06
CHEMBL4638548 1 8.71
CHEMBL4638345 1 8.08
CHEMBL2111101 PIMAVANSERIN 4.0 1 7.66
CHEMBL4633971 1 7.46
CHEMBL4649679 1 6.50
CHEMBL4640741 1 5.95
CHEMBL4639499 1 5.70
CHEMBL4645022 1 -
CHEMBL4633476 1 -
CHEMBL4642682 1 -
CHEMBL4636657 1 -
CHEMBL4646280 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4635010 IC50 = 0.39 nM 9.41
CHEMBL4644559 IC50 = 0.6 nM 9.22
CHEMBL4647697 IC50 = 0.87 nM 9.06
CHEMBL4638548 IC50 = 1.93 nM 8.71
CHEMBL4638345 IC50 = 8.35 nM 8.08
CHEMBL2111101 PIMAVANSERIN IC50 = 22.0 nM 7.66
CHEMBL4633971 IC50 = 34.65 nM 7.46
CHEMBL4649679 IC50 = 317.3 nM 6.50
CHEMBL4640741 IC50 = 1126.0 nM 5.95
CHEMBL4639499 IC50 = 2018.0 nM 5.70
CHEMBL4645022 IC50 - -
CHEMBL4633476 IC50 - -
CHEMBL4642682 IC50 - -
CHEMBL4636657 IC50 - -
CHEMBL4646280 IC50 - -