Assay Detail
Binding
CHEMBL4616486
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of eIF4A1 in human MDA-MB-231 cells assessed as inhibition of cellular-translation incubated for 4 hrs by specific tandem sequence motif repeat AGAGAG in 5'- UTR containing luciferase reporter gene assay
5
Total Activities
4
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | MDA-MB-231 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Eukaryotic initiation factor 4A-I (CHEMBL2052028) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Design of Development Candidate eFT226, a First in Class Inhibitor of Eukaryotic Initiation Factor 4A RNA Helicase.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 7.54 | 8.82 |
| Inhibition | 1 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4303782 | ZOTATIFIN | 2.0 | 2 | 8.82 |
| CHEMBL438139 | ROCAGLAMIDE | — | 1 | 8.33 |
| CHEMBL123292 | CYCLOHEXIMIDE | 2.0 | 1 | 6.65 |
| CHEMBL1098427 | HIPPURISTANOL | — | 1 | 6.37 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4303782 | ZOTATIFIN | IC50 | = | 1.5 | nM | 8.82 |
| CHEMBL438139 | ROCAGLAMIDE | IC50 | = | 4.7 | nM | 8.33 |
| CHEMBL123292 | CYCLOHEXIMIDE | IC50 | = | 226.0 | nM | 6.65 |
| CHEMBL1098427 | HIPPURISTANOL | IC50 | = | 426.0 | nM | 6.37 |
| CHEMBL4303782 | ZOTATIFIN | Inhibition | - | % | - |