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Assay Detail

CHEMBL4616985

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N terminal His6-tagged Escherichia coli BL21(DE3) beta-glucuronidase using IPTG as substrate after 4 hrs
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli BL21(DE3)
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Therapeutic significance of β-glucuronidase activity and its inhibitors: A review.
Awolade P, Cele N, Kerru N, Gummidi L, Oluwakemi E, Singh P.
Eur J Med Chem (2020) 187 : 111921 -111921
PubMed 31835168 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 6.95 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4635113 1 7.70
CHEMBL4636654 1 7.52
CHEMBL4633508 1 7.52
CHEMBL4635884 1 7.52
CHEMBL4647420 1 7.40
CHEMBL4649028 1 7.40
CHEMBL4632883 1 7.30
CHEMBL4634028 1 7.05
CHEMBL4644220 1 7.00
CHEMBL4642704 1 6.92
CHEMBL4643487 1 6.89
CHEMBL4643998 1 6.52
CHEMBL4643027 1 6.48
CHEMBL4645207 1 6.06
CHEMBL4644433 1 4.99
CHEMBL4648659 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4635113 IC50 = 20.0 nM 7.70
CHEMBL4636654 IC50 = 30.0 nM 7.52
CHEMBL4633508 IC50 = 30.0 nM 7.52
CHEMBL4635884 IC50 = 30.0 nM 7.52
CHEMBL4647420 IC50 = 40.0 nM 7.40
CHEMBL4649028 IC50 = 40.0 nM 7.40
CHEMBL4632883 IC50 = 50.0 nM 7.30
CHEMBL4634028 IC50 = 90.0 nM 7.05
CHEMBL4644220 IC50 = 100.0 nM 7.00
CHEMBL4642704 IC50 = 120.0 nM 6.92
CHEMBL4643487 IC50 = 130.0 nM 6.89
CHEMBL4643998 IC50 = 300.0 nM 6.52
CHEMBL4643027 IC50 = 330.0 nM 6.48
CHEMBL4645207 IC50 = 880.0 nM 6.06
CHEMBL4644433 IC50 = 10170.0 nM 4.99
CHEMBL4648659 IC50 <= 0.02 nM -