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Assay Detail

CHEMBL4617680

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant Escherichia coli NDM-1 using imipenem as substrate preincubated for 10 mins followed by substrate addition and measured every 30 secs for 30 mins by spectrophotometric analysis
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery of Cyclic Boronic Acid QPX7728, an Ultrabroad-Spectrum Inhibitor of Serine and Metallo-β-lactamases.
Hecker SJ, Reddy KR, Lomovskaya O, Griffith DC, Rubio-Aparicio D, Nelson K, Tsivkovski R, Sun D, Sabet M, Tarazi Z, Parkinson J, Totrov M, Boyer SH, Glinka TW, Pemberton OA, Chen Y, Dudley MN.
J Med Chem (2020) 63 : 7491 -7507
PubMed 32150407 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 5 6.75 7.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4640702 1 7.62
CHEMBL4633785 XERUBORBACTAM 1.0 1 7.50
CHEMBL4636953 1 5.13
CHEMBL1689063 AVIBACTAM 4.0 1 -
CHEMBL3317857 VABORBACTAM 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4640702 Ki = 24.0 nM 7.62
CHEMBL4633785 XERUBORBACTAM Ki = 32.0 nM 7.50
CHEMBL4636953 Ki = 7400.0 nM 5.13
CHEMBL1689063 AVIBACTAM Ki > 40000.0 nM -
CHEMBL3317857 VABORBACTAM Ki > 40000.0 nM -