Assay Detail

CHEMBL4617682

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Binding

Inhibition of recombinant Escherichia coli TEM-10 using nitrocefin as substrate preincubated for 10 mins followed by substrate addition measured every 10 secs for 10 mins by spectrophotometrically analysis

ChEMBL ↗

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Target

Unchecked (CHEMBL612545) ↗

Open DB target context

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Unchecked (CHEMBL612545) is the working anchor for this assay review. Loading the live Open DB target snapshot…

Primary target Unchecked

CHEMBL612545

Anchored from Assay target.

Protein identity Unchecked

Disease frame Disease evidence pending

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Review focus Open DB context pending

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Total Activities

Compounds Tested

Activity Types

Assay Parameters

Assay Information

Assay Type	Binding
Organism	Escherichia coli
Confidence	0 — Uncurated / Unknown
Curated By	Autocuration

Target

Unchecked (CHEMBL612545) ↗

Type

UNCHECKED

Publication

Discovery of Cyclic Boronic Acid QPX7728, an Ultrabroad-Spectrum Inhibitor of Serine and Metallo-β-lactamases.

Hecker SJ, Reddy KR, Lomovskaya O, Griffith DC, Rubio-Aparicio D, Nelson K, Tsivkovski R, Sun D, Sabet M, Tarazi Z, Parkinson J, Totrov M, Boyer SH, Glinka TW, Pemberton OA, Chen Y, Dudley MN.
J Med Chem (2020) 63 : 7491 -7507
PubMed 32150407 · DOI

Activity Statistics

Type	Count	Avg pChEMBL	Best pChEMBL
Ki	3	8.29	9.18

Compounds Tested

Compound	Name	Phase	Activities	Best pChEMBL
CHEMBL4633785	XERUBORBACTAM	1.0	1	9.18
CHEMBL1689063	AVIBACTAM	4.0	1	8.85
CHEMBL3317857	VABORBACTAM	4.0	1	6.85

Activity Data

Compound	Name	Type	Rel.	Value	Units	pChEMBL
CHEMBL4633785	XERUBORBACTAM	Ki	=	0.66	nM	9.18
CHEMBL1689063	AVIBACTAM	Ki	=	1.4	nM	8.85
CHEMBL3317857	VABORBACTAM	Ki	=	140.0	nM	6.85