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Assay Detail

CHEMBL4618933

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal FLAG-tagged EZH2 in PRC2 complex (unknown origin) expressed in baculovirus infected Sf9 cells using H2N-RKQLATKAAR(Kme0)SAPATGGVKKP-NTPEGBiot peptide as substrate preincubated with [3H]SAM for 4 to 5 hrs followed by substrate addition and measured after 20 hrs by Topcount reader analysis
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone-lysine N-methyltransferase EZH2 (CHEMBL2189110)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, Synthesis, and Pharmacological Evaluation of Second Generation EZH2 Inhibitors with Long Residence Time.
Khanna A, Côté A, Arora S, Moine L, Gehling VS, Brenneman J, Cantone N, Stuckey JI, Apte S, Ramakrishnan A, Bruderek K, Bradley WD, Audia JE, Cummings RT, Sims RJ, Trojer P, Levell JR.
ACS Med Chem Lett (2020) 11 : 1205 -1212
PubMed 32551002 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 9.65 10.24

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4639983 1 10.24
CHEMBL4640994 1 10.23
CHEMBL4638754 1 10.22
CHEMBL4637572 1 10.20
CHEMBL4635809 1 10.17
CHEMBL4635863 1 10.14
CHEMBL4635102 1 10.04
CHEMBL4633923 1 10.00
CHEMBL4639913 1 10.00
CHEMBL4633932 1 9.92
CHEMBL4634677 1 9.89
CHEMBL4637958 1 9.85
CHEMBL4649131 1 9.80
CHEMBL4639569 1 9.74
CHEMBL4639616 1 9.74
CHEMBL4632988 1 9.70
CHEMBL4633053 1 9.70
CHEMBL4644363 1 9.70
CHEMBL4641325 1 9.70
CHEMBL4634390 1 9.31
CHEMBL4638163 1 9.21
CHEMBL4639975 1 9.14
CHEMBL4638720 1 8.42
CHEMBL4634535 1 8.39
CHEMBL4633598 1 7.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4639983 IC50 = 0.057 nM 10.24
CHEMBL4640994 IC50 = 0.059 nM 10.23
CHEMBL4638754 IC50 = 0.06 nM 10.22
CHEMBL4637572 IC50 = 0.063 nM 10.20
CHEMBL4635809 IC50 = 0.067 nM 10.17
CHEMBL4635863 IC50 = 0.072 nM 10.14
CHEMBL4635102 IC50 = 0.092 nM 10.04
CHEMBL4633923 IC50 = 0.1 nM 10.00
CHEMBL4639913 IC50 = 0.1 nM 10.00
CHEMBL4633932 IC50 = 0.12 nM 9.92
CHEMBL4634677 IC50 = 0.13 nM 9.89
CHEMBL4637958 IC50 = 0.14 nM 9.85
CHEMBL4649131 IC50 = 0.16 nM 9.80
CHEMBL4639569 IC50 = 0.18 nM 9.74
CHEMBL4639616 IC50 = 0.18 nM 9.74
CHEMBL4632988 IC50 = 0.2 nM 9.70
CHEMBL4633053 IC50 = 0.2 nM 9.70
CHEMBL4644363 IC50 = 0.2 nM 9.70
CHEMBL4641325 IC50 = 0.2 nM 9.70
CHEMBL4634390 IC50 = 0.49 nM 9.31
CHEMBL4638163 IC50 = 0.62 nM 9.21
CHEMBL4639975 IC50 = 0.73 nM 9.14
CHEMBL4638720 IC50 = 3.8 nM 8.42
CHEMBL4634535 IC50 = 4.1 nM 8.39
CHEMBL4633598 IC50 = 19.0 nM 7.72