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Assay Detail

CHEMBL4618934

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of EZH2 in human HeLa cells assessed as inhibition of trimethylation of H3K27 after 72 hrs by AlphaLISA assay
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone-lysine N-methyltransferase EZH2 (CHEMBL2189110)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, Synthesis, and Pharmacological Evaluation of Second Generation EZH2 Inhibitors with Long Residence Time.
Khanna A, Côté A, Arora S, Moine L, Gehling VS, Brenneman J, Cantone N, Stuckey JI, Apte S, Ramakrishnan A, Bruderek K, Bradley WD, Audia JE, Cummings RT, Sims RJ, Trojer P, Levell JR.
ACS Med Chem Lett (2020) 11 : 1205 -1212
PubMed 32551002 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 24 8.22 9.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4639913 1 9.15
CHEMBL4639983 1 8.74
CHEMBL4638754 1 8.74
CHEMBL4637958 1 8.74
CHEMBL4640994 1 8.70
CHEMBL4649131 1 8.62
CHEMBL4639616 1 8.60
CHEMBL4637572 1 8.47
CHEMBL4635102 1 8.46
CHEMBL4635863 1 8.46
CHEMBL4633932 1 8.29
CHEMBL4639569 1 8.23
CHEMBL4633923 1 8.20
CHEMBL4634677 1 8.17
CHEMBL4635809 1 7.58
CHEMBL4297463 CPI-1205 1.0 1 7.55
CHEMBL4639975 1 7.34
CHEMBL4634390 1 7.31
CHEMBL4638163 1 6.74
CHEMBL4634535 1 -
CHEMBL4633598 1 -
CHEMBL4633053 1 -
CHEMBL4644363 1 -
CHEMBL4632988 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4639913 EC50 = 0.7 nM 9.15
CHEMBL4639983 EC50 = 1.8 nM 8.74
CHEMBL4638754 EC50 = 1.8 nM 8.74
CHEMBL4637958 EC50 = 1.8 nM 8.74
CHEMBL4640994 EC50 = 2.0 nM 8.70
CHEMBL4649131 EC50 = 2.4 nM 8.62
CHEMBL4639616 EC50 = 2.5 nM 8.60
CHEMBL4637572 EC50 = 3.4 nM 8.47
CHEMBL4635102 EC50 = 3.5 nM 8.46
CHEMBL4635863 EC50 = 3.5 nM 8.46
CHEMBL4633932 EC50 = 5.1 nM 8.29
CHEMBL4639569 EC50 = 5.9 nM 8.23
CHEMBL4633923 EC50 = 6.3 nM 8.20
CHEMBL4634677 EC50 = 6.7 nM 8.17
CHEMBL4635809 EC50 = 26.0 nM 7.58
CHEMBL4297463 CPI-1205 EC50 = 28.0 nM 7.55
CHEMBL4639975 EC50 = 45.3 nM 7.34
CHEMBL4634390 EC50 = 48.5 nM 7.31
CHEMBL4638163 EC50 = 181.0 nM 6.74
CHEMBL4634535 EC50 > 304.0 nM -
CHEMBL4633598 EC50 > 304.0 nM -
CHEMBL4633053 EC50 > 1000.0 nM -
CHEMBL4644363 EC50 > 1000.0 nM -
CHEMBL4632988 EC50 < 100.0 nM -