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Assay Detail

CHEMBL4619095

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Modulation of gamma secretase in human H4 cells overexpressing human APP695 harboring K595N/M596L Swedish double mutant assessed as reduction in total amyloid beta (1 to 42 residues) production measured after 24 hrs by AlphaLISA assay
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Gamma-secretase (CHEMBL2094135)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery of RO7185876, a Highly Potent γ-Secretase Modulator (GSM) as a Potential Treatment for Alzheimer's Disease.
Ratni H, Alker A, Bartels B, Bissantz C, Chen W, Gerlach I, Limberg A, Lu M, Neidhart W, Pichereau S, Reutlinger M, Rodriguez-Sarmiento RM, Jakob-Roetne R, Schmitt G, Zhang E, Baumann K.
ACS Med Chem Lett (2020) 11 : 1257 -1268
PubMed 32551009 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 31 7.56 8.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4645821 1 8.05
CHEMBL4525398 1 7.96
CHEMBL4634277 1 7.85
CHEMBL4646084 1 7.85
CHEMBL4634813 1 7.85
CHEMBL4649661 1 7.85
CHEMBL4635678 1 7.82
CHEMBL4638029 1 7.82
CHEMBL4638559 1 7.80
CHEMBL4636270 1 7.72
CHEMBL4647270 1 7.72
CHEMBL4645645 1 7.64
CHEMBL4638982 1 7.64
CHEMBL4644229 1 7.60
CHEMBL4644454 1 7.60
CHEMBL4646041 1 7.58
CHEMBL4647524 1 7.54
CHEMBL4633835 1 7.52
CHEMBL4637328 1 7.52
CHEMBL4633563 1 7.51
CHEMBL4635797 1 7.51
CHEMBL4634104 1 7.50
CHEMBL4643195 1 7.47
CHEMBL4645134 1 7.41
CHEMBL4634876 1 7.40
CHEMBL4648911 1 7.36
CHEMBL4649500 1 7.36
CHEMBL4632791 1 7.31
CHEMBL4646136 1 7.06
CHEMBL4634397 1 6.90

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4645821 IC50 = 9.0 nM 8.05
CHEMBL4525398 IC50 = 11.0 nM 7.96
CHEMBL4634277 IC50 = 14.0 nM 7.85
CHEMBL4646084 IC50 = 14.0 nM 7.85
CHEMBL4634813 IC50 = 14.0 nM 7.85
CHEMBL4649661 IC50 = 14.0 nM 7.85
CHEMBL4635678 IC50 = 15.0 nM 7.82
CHEMBL4638029 IC50 = 15.0 nM 7.82
CHEMBL4638559 IC50 = 16.0 nM 7.80
CHEMBL4636270 IC50 = 19.0 nM 7.72
CHEMBL4647270 IC50 = 19.0 nM 7.72
CHEMBL4645645 IC50 = 23.0 nM 7.64
CHEMBL4638982 IC50 = 23.0 nM 7.64
CHEMBL4644229 IC50 = 25.0 nM 7.60
CHEMBL4644454 IC50 = 25.0 nM 7.60
CHEMBL4646041 IC50 = 26.0 nM 7.58
CHEMBL4647524 IC50 = 29.0 nM 7.54
CHEMBL4633835 IC50 = 30.0 nM 7.52
CHEMBL4637328 IC50 = 30.0 nM 7.52
CHEMBL4633563 IC50 = 31.0 nM 7.51
CHEMBL4635797 IC50 = 31.0 nM 7.51
CHEMBL4634104 IC50 = 32.0 nM 7.50
CHEMBL4643195 IC50 = 34.0 nM 7.47
CHEMBL4645134 IC50 = 39.0 nM 7.41
CHEMBL4634876 IC50 = 40.0 nM 7.40
CHEMBL4648911 IC50 = 44.0 nM 7.36
CHEMBL4649500 IC50 = 44.0 nM 7.36
CHEMBL4632791 IC50 = 49.0 nM 7.31
CHEMBL4646136 IC50 = 88.0 nM 7.06
CHEMBL4634397 IC50 = 126.0 nM 6.90
CHEMBL4636263 IC50 = 237.0 nM 6.62