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Assay Detail

CHEMBL4620552

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GFP-tagged KRAS G12V mutant (unknown origin) expressed in MDCK cells cotransfected with mCherry-CAXX assessed as increase in KRAS G12V-plasma membrane dissociation incubated for 48 hrs by quantitative confocal microscopic method
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type MDCK
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

GTPase KRas (CHEMBL2189121)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Chalcones bearing a 3,4,5-trimethoxyphenyl motif are capable of selectively inhibiting oncogenic K-Ras signaling.
Kovar SE, Fourman C, Kinstedt C, Williams B, Morris C, Cho KJ, Ketcha DM.
Bioorg Med Chem Lett (2020) 30 : 127144 -127144
PubMed 32276831 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 4.94 5.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL598038 1 5.15
CHEMBL62070 1 5.13
CHEMBL4646677 1 5.13
CHEMBL4643559 1 5.09
CHEMBL59351 1 5.09
CHEMBL187437 1 5.08
CHEMBL471655 1 5.04
CHEMBL59026 1 4.80
CHEMBL589993 1 4.61
CHEMBL3219765 1 4.28

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL598038 IC50 = 7010.0 nM 5.15
CHEMBL62070 IC50 = 7420.0 nM 5.13
CHEMBL4646677 IC50 = 7490.0 nM 5.13
CHEMBL4643559 IC50 = 8200.0 nM 5.09
CHEMBL59351 IC50 = 8200.0 nM 5.09
CHEMBL187437 IC50 = 8290.0 nM 5.08
CHEMBL471655 IC50 = 9170.0 nM 5.04
CHEMBL59026 IC50 = 15900.0 nM 4.80
CHEMBL589993 IC50 = 24640.0 nM 4.61
CHEMBL3219765 IC50 = 52970.0 nM 4.28