Assay Detail
Binding
CHEMBL4621228
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of Clostridium histolyticum ColH peptidase domain expressed in Escherichia coli BL21(DE3) cells using Mca-Ala-Gly-Pro-Pro-Gly-Pro-Dpa-Gly-Arg-NH2 as substrate preincubated for 1 hr followed by substrate addition measured for 2 mins by FRET assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Hathewaya histolytica |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
<i>N</i>-Aryl-3-mercaptosuccinimides as Antivirulence Agents Targeting <i>Pseudomonas aeruginosa</i> Elastase and <i>Clostridium</i> Collagenases.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 7.16 | 7.77 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4640494 | — | — | 1 | 7.77 |
| CHEMBL4636059 | — | — | 1 | 7.22 |
| CHEMBL4637937 | — | — | 1 | 6.50 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4640494 | — | IC50 | = | 17.0 | nM | 7.77 |
| CHEMBL4636059 | — | IC50 | = | 60.0 | nM | 7.22 |
| CHEMBL4637937 | — | IC50 | = | 320.0 | nM | 6.50 |